estimation of methionine and lysine in protein by thin-layer chromatography
نویسندگان
چکیده
a thin-layer chromatography method has been developed and evaluated for the separation and determination of lysine and methonine in a mixture of amino acids obtained from acid or enzyme hydrolysis of protein. the solvent systems were (a) ethanol-ether, (b) phenol-water, (c) ethyl methyl ketone-pyridine-distilled water-glacial acetic acid. procedures in all the three stages were in the same dimension. results of casein analysis by this method showed ± 15% fluctuations when compared with standard amino acid analyzer.
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عنوان ژورنال:
iranian journal of public healthجلد ۱۲، شماره ۱-۴، صفحات ۳۲-۴۲
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